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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: Food Chem. 2011 Sep 15;128(2):513–519. doi: 10.1016/j.foodchem.2011.03.073

Figure 3. Time course of kaempferol-induced apoptosis in A2780/CP70 ovarian cancer cells.

Figure 3

A2780/CP70 ovarian cancer cells were seeded in 96-well plates at 10,000 cells/well and incubated overnight. The cells were treated with 0- or 80-μM kaempferol in triplicates for 0, 2, 4, or 8 hours. No-cell wells were included for background correction. At each time point, a plate was removed of culture medium and frozen in -80 °C until final analysis. Cells were thawed, lyzed with Passive Lysis Bufer, and analyzed for caspase 3/7 activities with a Caspase-Glo 3/7 Assay and total protein levels with a BCA assay. Caspase 3/7 activities were normalized by total protein levels, and the levels of kaempferol-treated cells were expressed as percentages of the controls for statistics. Data represent Means ± SE from 2 independent experiments.