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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: Food Chem. 2011 Sep 15;128(2):513–519. doi: 10.1016/j.foodchem.2011.03.073

Figure 4. Kaempferol induces apoptosis in ovarian cancer cells.

Figure 4

Ovarian cancer cells were seeded in two 96-well plates at 10,000 cells/well and incubated overnight before treatment with kaempferol in triplicate for 2 hours. Plates were removed of culture medium and frozen at -80 °C. For apoptosis analysis, cells were thawed and lyzed with Passive Lysis Buffer, and measured for caspase 3/7 activities and total protein levels. No-cell wells were included for background correction. Caspase 3/7 activities were adjusted by total protein levels and expressed as percentages of the controls. Data represent Means ± SE from 6 or 7 independent experiments. *p<0.05, **p<0.01 as compared to control.