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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: Food Chem. 2011 Sep 15;128(2):513–519. doi: 10.1016/j.foodchem.2011.03.073

Figure 5. Kaempferol induces apoptosis in immortalized normal ovarian cells only at high concentration.

Figure 5

Immotalized normal ovarian epithelial cell line IOSE 364 were seeded in 96-well plates at 10,000 cells/well and incubated overnight. The cells were treated with various concentrations of kaempferol in triplicates for 2 hours and no-cell wells were included for background correction. Plates were then removed of culture medium and frozen at -80 °C. For apoptosis analysis, cells were thawed and lyzed with Passive Lysis Buffer, and measured for caspase 3/7 activities and total protein levels. Caspase 3/7 activities were normalized by total protein levels and expressed as percentages of controls. Data represent Means ± SE from 3 independent experiments. *p<0.05 as compared to control.