Fluorescence emission spectra for all the FM-labeled CYP2B4 variants (0.05 µM) in the presence of 3 µM CPR, 0.1 mg/ml DLPC at 23°C, pH 7.4. When reconstituted with excess CPR, the proximal variants showed the greatest increases in fluorescence enhancement, the distal variants showed the lowest enhancement, while the variants on regions perpendicular to the heme-thiolate ligand exhibited enhancements that were intermediate between the proximal and distal variants.