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Determination of the apparent Km and kcat values of the CYP2B4 (A) proximal, (B) side and (C) distal variants.The N-demethylation of benzphetamine to produce formaldehyde (HCHO) was measured at a constant concentration of CYP2B4 (0.2 µM) with increasing concentrations of CPR as described under “Experimental Procedures”. Error bars are the standard deviations from three measurements.
