Skip to main content
. Author manuscript; available in PMC: 2012 May 17.
Published in final edited form as: Biochemistry. 2011 Apr 22;50(19):3957–3967. doi: 10.1021/bi1020748

Table 3. The rate constants and amplitudes observed for the reduction of CYP2B4 and its variants by CPR at 23°C.

The apparent rate constants for the two kinetic phases are k1 and k2, respectively, and the relative amplitudes for each phase are expressed as A1% and A2%. The total absolute amplitude for the overall reduction is AT.

2B4 Kinetic parameters
AT A1% k1,S−1 A2% k2,S−1
WT 0.110 62 0.70 39 0.16
C79SC152S 0.110 28 0.51 72 0.067
C79S 0.079 18 0.205 82 0.052
(E60C) 0.120 28 0.678 72 0.077
(H226C) 0.061 33 0.15 67 0.043
(Y484C) 0.079 21 0.212 79 0.049
(V267C) 0.027 64 0.057 36 0.0138
(R133C) 0.021 85 0.019 15 0.0031
(L270C) 0.029 10 0.032 90 0.0110
(L420C) 0.070 39 0.360 61 0.0035