Fig. 9. Comparison of the effect of deletions and insertions within the α inter-domain linker on transcription from CRP-dependent promoters in vitro and in vivo. (A) Amount of transcripts produced by RNAP reconstituted with each mutant α subunit in the presence of CRP at the CC(–41.5) (▴) and CC(–61.5) (○) promoters in vitro. Data are taken from Figure 3. (B) Activity of the CC(–41.5) (▴) and CC(–61.5) (○) promoters in cells expressing chromosome-encoded wild-type α and plasmid-encoded mutant or wild-type α. Data are taken from Figure 8. Values in both panels are expressed as a percentage of the promoter activity in the presence of RNAP containing only wild-type α.