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HSL activation in 3T3-L1 adipocytes. Cells plated in a 96-well dish were exposed to the control medium (A) and (C) or 10 μM ISO for 15 min (B) and (D), and then fixed and labeled for nuclei, lipid droplets (green), and protein (red). (A, B) Cells observed for HSL. (C, D) Cells observed for pHSL-ser660. Images were collected with a 40 × objective. Scale bar = 50 μm. HSL, hormone sensitive lipase.
