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. 2011 Jun;9(3):262–280. doi: 10.1089/adt.2010.0302

Fig. 5.

Fig. 5.

Effects of lipolytic agents on pHSLser660 in subpopulations of 3T3L1 cells. 3T3L1 adipocytes were exposed to the indicated concentrations (μM) of ANP, ISO, FSK, and Lys-γ3-MSH and assayed for pHSLser660. The cells were then fixed and observed for nuclei, lipid droplets, and pHSLser660 and scanned using a 20 × objective (four images per well, an average of 481 cells/well). Data from the total population of cells are shown as white bars. Subpopulations of cells with a small degree of lipid droplet content (Small Lm, gray bars) or a high degree of lipid droplet content (Large Lm, black bars) were identified by CyteSeer utilizing the criteria of Table 3. (A–E) represent Area Pm, API Pi Pm, M Pr, M1, and Tan, respectively, and each bar is the mean ± SD for n = 16 wells. Within each population of cells the effects of ISO, FSK, and Lys-γ-MSH were significant versus control at P < 0.001 (asterisks were omitted for clarity). aDifferent from the Total population and the Small Lm subpopulation (P < 0.05); bdifferent from the Small Lm subpopulation (P < 0.05); cdifferent from the Total population (P < 0.05). ANP, atrial natriuretic peptide; FSK, Forskolin.