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. 2011 Mar 4;30(8):1634–1644. doi: 10.1038/emboj.2011.62

Figure 3.

Figure 3

OVA257−264 presentation from Tac-based proteins via the classical MHC class I pathway. (A) OVA257−264 presentation from L-Kb cells infected with the indicated viruses at 3 p.f.u./cell for 5 h in the presence or absence of epoxomicin (3 μM). A vaccinia virus that expresses the SIINFEKL-encoding minigene at limiting amounts via a 5′ UTR hairpin technique (m/S-HP18) (Wherry et al, 1999) was used as a toxicity control to confirm that there was no effect upon proteasome-independent presentation. Data are representative of three independent experiments. (B) OVA257−264 presentation from TAP-deficient B6 fibroblast cells (H-2b haplotype) infected with the indicated viruses at 3 p.f.u./cell for 5 h. A vaccinia virus that expresses the SIINFEKL-encoding minigene targeted to the ER independent of TAP function via signal sequence-mediated translocation, ss-m/S, was used as a control to show processing and presentation capability via a TAP-independent pathway. Data are representative of three independent experiments.