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. 2011 Mar 4;30(8):1634–1644. doi: 10.1038/emboj.2011.62

Figure 6.

Figure 6

Transfer of the Tac TM domain to another model antigen. (A) NP13−498-based model antigens. HA, HA-tag; S, OVA257−264; TM, Tac transmembrane. Right panel, metabolic pulse chase of NP13−498-based proteins. (B) Stability of the model antigens quantified by phosphoroimaging analysis. Data represent the mean and s.d. from three independent experiments. (C) OVA257−264 presentation from NP13−498-based proteins in a 5-h infection assay. Error bars represent the s.d. of triplicate samples. This experiment was repeated three times with similar results. (D) Metabolic pulse chase of N-end rule substrate of NP13−498 protein, Ub-R-NP13−498. Bottom panel, stability of Ub-R-NP13−498 quantified by phosphoroimaging analysis. Data represent the mean and s.d. from two independent experiments. (E) OVA257−264 presentation from NP13−498 proteins appended with a degradation signal (TM or N-degron) in a 5-h infection assay. Error bars represent the s.d. of triplicate samples. This experiment was repeated three times with similar results.