Skip to main content
. 2011 Mar 18;30(8):1645–1658. doi: 10.1038/emboj.2011.68

Figure 1.

Figure 1

High intracellular [CO2] and [HCO3−] activate S-type anion channel currents in Arabidopsis ca1;ca4 double-mutant guard cells but do not activate S-type anion currents in slac1 mutant guard cells with 2 μM [Ca2+]i. (A) Whole-cell currents without HCO3−/CO2 and (B) with 11.5 mM free [HCO3−]i/2 mM free CO2 in the pipette solution (pH 7.1) in ca1;ca4 double-mutant guard cells. (C) Steady-state current–voltage relationships of the whole-cell currents recorded in ca1;ca4 mutant guard cells as in (A) (open circles, n=4 guard cells) and (B) (filled circles, n=9 guard cells). (D) Steady-state current–voltage relationships of whole-cell currents recorded in slac1-1 mutant guard cells (open circles: 0 mM added [HCO3−]i, n=6; filled circles: 11.5 mM free [HCO3−]i and 2 mM free [CO2], n=6) and (E) in slac1-3 mutant guard cells (open circles: 0 mM added [HCO3−]i, n=4; filled circles: 11.5 mM free [HCO3−]i and 2 mM free [CO2], n=8). Liquid junction potential was +1 mV. Data are mean±s.e.