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. 2011 Mar 18;30(8):1645–1658. doi: 10.1038/emboj.2011.68

Figure 6.

Figure 6

HCO3−/CO2 activation S-type anion channel currents is disrupted in ost1-2 and ost1-3 mutant guard cells with 2 μM [Ca2+]i. (A) Whole-cell recording without and (B) with 13.5 mM total bicarbonate (11.5 mM free [HCO3−]i + 2 mM free [CO2]) added to the pipette solution in ost1-2 mutant guard cells. (C) Whole-cell recording with 13.5 mM total bicarbonate in the pipette solution in ost1-3 mutant guard cells. (D) Whole-cell currents with 13.5 mM total bicarbonate and (E) without bicarbonate added to the pipette solution in wild-type Ler guard cell protoplasts. (F) Steady-state current–voltage relationships of recordings as in (A) (open squares: ost1-2, –[HCO3−]i, n=5), (B) (filled squares: ost1-2, +[HCO3−]i, n=6), (C) (filled triangles: ost1-3, +[HCO3−]i, n=6), (D) (filled circles: wild-type Ler, +[HCO3−]i, n=7) and (E) (open circles: wild-type Ler, –[HCO3−]i, n=5). The pipette solution was adjusted to pH 7.1 in all the recordings. Liquid junction potential was +1 mV. Data are mean±s.e.