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. 2011 Mar 4;30(8):1577–1592. doi: 10.1038/emboj.2011.59

Figure 10.

Figure 10

N-cadherin is not required for AMPAR retrograde signalling in presynaptic maturation. (A) Immunoblot of endogenous N-cadherin from lysate of 14 DIV hippocampal culture and HEK293 cells. (B) N-cadherin RNAi delivered with lentivirus had a 94% knockdown efficiency in hippocampal cultures (n=3 experiments). (C) Representative images of N-cadherin knockdown in neuronal dendrites (red: N-cadherin; green: MAP2). Scale bar: 10 μm. (D) Quantification of the number of inactive glutamatergic synapses formed on HEK293 cells normalized to neighbouring neuronal synapses (n=13–15 cells/group; *P<0.05; **P<0.01; ***P<0.005). (E) Representative images of 12 DIV neurons labelled with Syt1 antibody uptake (red) and postfixation VGluT1 immunostaining (blue). Neuronal dendrites were identified by MAP2 immunostaining (green). Arrows indicate active glutamate release sites. Scale bar: 10 μm. (F) Quantification of the mean Syt1 puncta intensity at active synapses and the percent of inactive presynaptic terminals (n=18 cells/group; P>0.2).