Table 1. Candidate centrosomal proteins identified by PCP-SILAC.

Gene name	Suggested new name	Localization			Turn over (%)	Substructure
		PCP-SILAC score	GFP	HPA
KIAA0562	CEP104	7.8	×		95	Centriole
IFFO2		4.7	—		94
CKAP2L		7.1	×		93	Spindle pole, spindle, midbody
MIB1		6.4	×	×	89	GFP: satellites, spindle poles HPA: centrosomal with some satellites
AKNA		2.7	—		86
CCDC21	CEP85	2.3	×	×	83	GFP: centrosomal, spindle poles HPA: nucleolar interphase, spindle poles
C6orf182		2.6	—		83
CCDC14		6.1		×	77	Satellites
FBXW11		3.0	×		75	Probably centrosomal, spindle pole
MAP7D3		4.1			74
MPHOSPH9		3.0	—	×	72	Centriole
Albatross		4.8		×	72	Mother centriole, spindle pole
SLAIN1		1.7	(×)	—	71	Weak spindle
CCDC123	CEP89	1.8	×	×	71	GFP: near centrosomes, spindle poles HPA: satellites
CCHCR1		2.5	—		71
CCDC102A		2.2	—		66
GARNL4		6.5		×	64	Centrosomal in some cells
CCDC34		2.4			64
C3orf34	CEP19	5.0	×		64	Preferentially mother centriole
C6orf204		1.9	(×)	×	62	GFP: weak midbody and spindle HPA: spindle poles, possibly centrosomal
KIAA1712	CEP44	3.0	×		60	Centrosomal (two dots), spindle poles, weak midbody, possibly spindle
ANKRD26		2.8	×		58	Centrosomal, spindle poles
SLAIN2		2.2	(×)	×	58	GFP: very weak spindle, weak midbody HPA: centrosomal, spindle poles
KIAA0753		3.5	×	×	56	GFP: satellites, spindle poles, some midbody HPA: near centrosomes
CCDC45	CEP45	3.9	×	×	54	GFP: weak HPA: centrosomal (two dots), spindle poles
C16orf63		6.2	×		50	Satellites around centrosome, spindle poles
TCHP		2.3	×		49	Spindle, midbody, possibly weak centrosomal
C1orf96		6.7	×		48	Midbody, possibly spindle and spindle poles
CCDC46	CEP112	4.1		×	48	Centrosomal (two dots), around spindle poles in some cells
C14orf145	CEP128	0.5		×	40	Mother centriole, spindle pole
ACTR1B		8.7	×		38	Spindle poles, possibly centrosomal
ACTR10		5.3			34
CCDC41		3.0	(×)		28
C7orf47		3.8			22
RTTN		6.4	—		21
NOG		3.8			19
PRKACB		3.3		×	NA	Satellites, possibly midbody
WDR90		3.3	—		NA
IIP45		5.0			NA
IRAK1BP1		6.0	—	—	NA
Novel proteins identified with low PCP-SILAC organelle classification score were validated by fluorescence microscopy using human protein atlas antibodies (HPA) or cell pools stable expressing fluorescently tagged proteins (GFP), where indicated. Protein turnover rates were calculated as the percentage of stable isotope labelling after 20 h. Novel factors are termed CEP and a number, where CEP stands for centrosomal protein and is followed by the Mr calculated from the full-length sequence. For additional information, see Supplementary Table S4.