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Temperature dependence of spin-lattice relaxation rates is shown for dithionite reduced [4Fe-4S]+ in R. sphaeroides ETF-QO. The circles are the average values for the native and mutant samples and the error bars reflect the standard deviations for the four proteins. The fit line is the sum of contributions from the Raman and Orbach processes. The Debye temperature was fixed at 100 K and the energy of the low-lying excited state was ΔE = 205 (140 cm−1).
