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. Author manuscript; available in PMC: 2011 May 26.

Published in final edited form as: Biochemistry. 2007 Dec 11;47(1):92–100. doi: 10.1021/bi701859s

Potentiometric titration curves are shown of the semiquinone for R. sphaeroides (○) native, ( ) Y501F, ( ) T525A, and ( ) Y501F/T525A ETF-QOs. The semiquinone spectra were recorded at 105 K to avoid saturations of the EPR signal and the midpoint potentials were calculated from Nernst equations described by Paulsen (41).

Inline graphic — Potentiometric titration curves are shown of the semiquinone for R. sphaeroides (○) native, ( ) Y501F, ( ) T525A, and ( ) Y501F/T525A ETF-QOs. The semiquinone spectra were recorded at 105 K to avoid saturations of the EPR signal and the midpoint potentials were calculated from Nernst equations described by Paulsen (41).