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. Author manuscript; available in PMC: 2011 May 26.
Published in final edited form as: Biochemistry. 2007 Dec 11;47(1):92–100. doi: 10.1021/bi701859s

Figure 8.

Figure 8

Inversion recovery signals for semiquinone in the enzymatically reduced native (top), Y501F (middle), and Y501F/T525A (bottom) samples of R. sphaeroides ETF-QO at 50 K. The curves are the sums of contributions from semiquinone with neighboring diamagnetic [4Fe-4S]2+ and paramagnetic [4Fe-4S]+. Differences in the inversion recovery curves result from the different populations of paramagnetic [4Fe-4S]+ present: native (~80%), Y501F (25%), and Y501F/T525A (0%). The dashed lines are simulated curves calculated with MENOSR for an average inter-spin distance of ~18.4 ± 1Å.