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. Author manuscript; available in PMC: 2011 May 26.
Published in final edited form as: Biochemistry. 2007 Dec 11;47(1):92–100. doi: 10.1021/bi701859s

Table 1.

Activity assays, iron/flavin ratios, midpoint potentials, and enzymatic titration values of native and mutant ETF-QO samples.

Sample Iron/− Flavin Ratio [4Fe4S]+1,+2 Em (mV) Q/•Q− Em1 (mV) •Q−/QH2 Em2 (mV) Reductive Titration Δε430 Quinone Reductase Activity (s Activity (s−1) Disproportionation Activity (s Activity (s−1)
wild type 4.0:1 +37 ± 7 +38 ± 12 −62 ± 16 10453 24.2 (100%) 8.3 (100%)
Y501F 4.0:1 −64 ± 5 +39 ± −56 ± 8 9616 8.9 (37%) 4.1 (51%)
T525A 4.1:1 −58 ± 6 +56 ± −54 ± 7 9328 8.5 (35%) 5.1 (62%)
Y501F/T525A 3.8:1 −128 ± 4 +50 ± 5 −55 ± 4 8433 1.8 (8%) 0.66 (8%)