. Author manuscript; available in PMC: 2011 May 26.

Published in final edited form as: Biochemistry. 2007 Dec 11;47(1):92–100. doi: 10.1021/bi701859s

Table 2.

g-values and linewidths (G) for [4Fe-4S]⁺ at 15 K and Semiquinone at 105 K

Sample	[4Fe4S]⁺	Linewidth^c	[4Fe4S]⁺	Linewidth^c	[4Fe4S]⁺	Linewidth^c	SQ^b
Sample	g_z^a	(G)	g_y^a	(G)	g_x^a	(G)	g
Wild Type	2.089	22	1.934	15	1.875	29	2.0036
Y501F	2.088	28	1.933	30	1.873	37	2.0036
T525A	2.097	35	1.913	30	1.857	48	2.0036
Y501F/T525A	2.095	33	1.921	35	1.868	51	2.0036

The average uncertainty of the iron-sulfur cluster g-values was ~± 0.003.

The average uncertainty of the semiquinone g-values was ~± 0.0005.

Peak-to-peak first derivative linewidths were simulated using MONMER. Average uncertainty of the linewidth was ± 2 G for g_z and g_x and ± 10 G for g_y.