. Author manuscript; available in PMC: 2011 May 26.

Published in final edited form as: Biochemistry. 2007 Dec 11;47(1):92–100. doi: 10.1021/bi701859s

Table 3.

Populations of paramagnetic species in wild type and mutant ETF-QOs samples.

Sample	Redox State	% SQ^a	% [4Fe-4S]⁺^b
Wild Type	SQ max	53 ± 7	86
	Dithionite	14	90
Y501F	SQ Max	52 ± 4	20
	Dithionite	11	76
T525A	SQ Max	44 ± 3	20
	Dithionite	13	98
Y501F/T525A	SQ max	44 ± 3	0
	Dithionite	4	55

Determined using double integration of the semiquinone CW spectra relative to tempol standard. The percentages are relative to the total protein concentration. Values are accurate to ±5%.

Determined using double integration of the [4Fe-4S]⁺ cluster CW spectra relative to CuEDTA standard. The percentages are an average from duplicate measurements at 15 and 18 K and are relative to the total protein concentration. Values accurate to ±10%.