Fig. 1. LHP1 is required for growth of smd1–1 cells at low temperatures. (A–D) Wild-type cells (SMD1 LHP1), cells lacking LHP1 (SMD1 lhp1::LEU2) and smd1–1 cells carrying either no LHP1 (smd1–1 lhp1::LEU2), chromosomal LHP1 (smd1–1 LHP1) or LHP1 on a centromeric plasmid [smd1–1 (pLHP1)] were streaked to single colonies on YPD agar and grown at 37°C (A), 30°C (B) and 16°C (C). The position of each strain is shown in (D). (E) Extracts were prepared from the strains and subjected to Western blotting. The asterisk denotes an unrelated protein detected by the anti-Lhp1p serum.