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. 2010 Aug 20;6(1):4. doi: 10.1007/s11671-010-9741-8

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Copyright ©2010 Chen et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Confocal laser scanning microscopy (CLSM) images of HeLa cells after 2 h incubation with coumarin-6-loaded5% DMAB-modified PLGA-TPGS nanoparticles at 37.0°C. The cells were stained by propidium iodide (red) and the coumarin-6-loaded nanoparticles are green. The cellular uptake is visualized by overlaying images obtained by white light, FITC filter and PI filter: upper-left image from FITC channel; upper-right image from transmitted light channel; lower-left image from PI channel; lower-right image from combined transmitted light channel, PI channel and FITC channel.