Fig. 5.

Activation of Ano1 in zero intracellular Ca²⁺. (A–C) Representative traces of WT Ano1 (A), ₄₄₄EEEE/AAAA₄₄₇ (B), and ΔEAVK (C) activated by V_m in nominally zero Ca²⁺. (D) Mean current density at +200 mV with zero intracellular Ca²⁺ plus 5 mM EGTA, zero intracellular Ca²⁺ and Mg²⁺ plus 5 mM EGTA (EGTA + 0 Mg), or zero Ca²⁺ plus 5 mM EGTA plus 1 mM BAPTA in both intracellular and extracellular solutions (EGTA + BAPTA). n = 5–11. (E) Steady-state I-V curve from cells dialyzed with zero Ca²⁺: nontransfected cells (control; □), WT Ano1 (), Δ₄₄₄EEEEEAVKD₄₅₂ (△), ΔEAVK (▽), and ₄₄₄EEEE/AAAA₄₄₇ (♢). (n = 4–11).