Fig. 4. mcm4 binds to chromatin during anaphase B. Cells from an asynchronous culture (P560) in YE were processed using the in situ chromatin binding procedure, after which cells were stained with anti-α-tubulin antibody. (A) The left images indicate cells in different stages of anaphase B, showing mitotic spindles (red) and chromatin-bound mcm4 (green). Bar = 10 μm. The right images show the corresponding DNA staining (DAPI). (B) Proportion of mitotic cells with mcm4-positive nuclei shown according to mitotic spindle length. On average, 12 cells were scored for each length class (range 5–20 cells).