Fig. 3.

Flow cytometry evidence that ERα is required in astrocytes, but not neurons, to reduce macrophage and T-cell inflammation. (A and C) Macrophages (R1) (CD11b^hi/CD45^hi) and (B and C) T-cells (CD45^hi/CD3^hi) were significantly reduced in WT, but not aCKO mice, treated with ERα ligand via flow cytometry from the CNS. (A and C) There were no significant differences among all groups in numbers of microglia (R2) (CD11b^hi/CD45^int). n = 5 per group. *P < 0.05. NS, not significant vs. WT+EAE+ERα ligand (ANOVA with post hoc Bonferroni pairwise analysis).