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. 2011 May 9;108(21):8698–8703. doi: 10.1073/pnas.1103802108

Fig. 1.

Fig. 1.

Inhibition of RAR signaling causes coloboma in zebrafish. (A) Coloboma (arrowheads) in embryos treated with 2.5–5 μM AGN from 3 to 6 s. (Left) Lateral view of 48-hpf phenylthiourea-treated embryos. (Center) Lateral (Center Left) and ventral (Center Right) views of 60-hpf eyes. (Right) Sections of 60-hpf eyes showing the lack of retinal pigmented epithelium (triangles) and a shortening of the retina ventral to the optic nerve head (arrows) in the AGN-treated embryo. (B, Left) Immunostaining with an anti-acetylated (Ac.) tubulin antibody at 48–50 hpf, showing abnormal retinal axon trajectories in the AGN-treated embryo (arrowheads), besides the normal contralateral projection (triangles). (Center) fli1a:EGFP transgenic embryos at 48 hpf. The AGN-treated embryo exhibits disrupted vascularization (triangles). (Right) sox10:EGFP transgenic embryos at 32–36 hpf. The AGN-treated embryo shows less POM cells within the choroid fissure (triangles).