Fig. 5.

PC activity predicts cellular independence from glutamine and glutaminase. (A, Left) Labeling of intracellular metabolites from D-[3,4-¹³C]-glucose in HepG2 and Huh-7 cells grown in complete medium, including 4 mM glutamine, for 7 h. (Right) Labeling of citrate from [1-¹³C]-pyruvate in HepG2 and Huh-7 cells grown in the presence and absence of glutamine for 7 h. (B) Viability and growth of HepG2 and Huh-7 cells in the presence and absence of glutamine for 72 h. (C) Protein expression in HepG2- and Huh-7-derived cell lines expressing shRNAs directed against GFP (control), GLS or PC (Top), and growth of each cell line in medium containing both glucose and glutamine. Data are the average ± SD of three independent cultures grown for 72 h. *P < 0.05, **P < 0.005.