Fig. 3. A/C mismatch repair by extracts of RH95021 cells. (A) An A/C mismatched substrate (141 fmol) was incubated for 20 min with extracts of Msh2^–/– RH95021 cells corresponding to the GFU indicated. The DNA was digested with MluI and analysed by agarose gel electrophoresis. The repair products (arrowed) were quantified as described in Materials and methods. (B) An A/C mismatched substrate was incubated with 1.0 GFU of cell extract for the times indicated. Products were analysed and quantified as above. RH95021 (▪); Colo26 (○).