Fig. 2.

Commensal bacteria oxidize and inactivate LMW-PTP. (A) Western blots of LWM-PTP levels in migrating T84 cells cultured in buffer containing biotin maleimide (BM) and treated with HBSS, LGG^lo (1.5 × 10⁷ cfu/mL), LGG^med (5.0 × 10⁷ cfu/mL), or LGG^hi (1.5 × 10⁸ cfu/mL) for the indicated times. Representative Western blots show LWM-PTP levels before (input; Lower) and after precipitation (pulldown, Upper). (B) Specific phosphatase activity of LMW-PTP–transfected HeLa cells treated with HBSS, LGG^lo (5.0 × 10⁷ cfu/mL), or LGG^hi (1.5 × 10⁸ cfu/mL) for the indicated times. Data are expressed as counts/mg of protein and are means ± SE of triplicate samples. Two independent experiments were performed and representative data from one experiment are shown.