Table 1.

Fitting Results for Three Equilibrium Constants

	K1>0, K2>0,K3>0	K1>0, K2>0,K3=0	K1>0, K2=0,K3>0
Equilibrium constantsa, mM-2
K1	0.206±0.0468	0.220±0.0499	0.106±0.0154
K2	0.0259±0.00841	0.0302±0.00820	0
K3	0.000716±0.000858	0	0.00386±0.000836
Goodness of fit
R2	0.899	0.897	0.782
ν	46	47	47
χ2	0.0837	0.0851	0.180
χν2	0.00182	0.00181	0.00383

^aThe mean equilibrium constants were determined by fitting the experimental data for the sample solutions with high concentrations of protein with Eq. (5). Every replicate was regarded as an individual data point during fitting. Data are presented as mean ± S.D.