Fig. 6.

Chromatic immunoprecipitation analysis of HNF4α associated with the UGT2B7 5′ flanking region. HepG2 cells were either transfected with an HNF4α expression vector or cotransfected with an activated CAR expression vector (VP-CAR). Transfected HepG2 cells were collected 48 h after the transfections. Cells were fixed and sonicated for the preparation of sheared chromatin, and immunoprecipitations were performed using HNF4α antibody, or nonspecific IgG, as a negative control. After immunoprecipitation, associated DNA was amplified with a pair of primers targeting UGT2B7 gene region −181 to +11, quantitated by real-time PCR, and displayed by gel electrophoresis. Input and Western blot of HNF4α indicate equal amounts of lysates used before immunoprecipitation.