Fig. 3.

A comparison of the rescue of C. elegans slo-1(js379) behavioral phenotypes by slo-1 and its human ortholog kcnma1. A, a comparison of reversal frequency for wild-type, slo-1(js379), and transgenic lines of slo-1(js379) expressing either slo-1 ▨ or kcnma1 ▩ behind the native promoter (pslo-1), a pan-neuronal promoter (psnb-1), or in body-wall muscle (pmyo-3). At least two stable lines for each js379;pslo-1::slo-1 and js379;pslo-1::kcnma1 transgenic strain were tested. All lines for the same strain showed comparable results, and the data are pooled. Data are the mean ± S.E.M. of n ≥20, ***, p < 0.001; **, p < 0.01; *, p < 0.05, one-way ANOVA with Bonferroni post hoc test. B, a comparison of the pattern of pharyngeal pumping in wild-type, slo-1(js379), and transgenic lines of slo-1(js379) expressing either slo-1 or kcnma1 behind the native promoter (pslo-1). Note the erratic pattern in slo-1(js379), which has a tendency to generate pharyngeal pumps in groups of three or more rather than as single evenly spaced pumps. This effect is ameliorated by expression of either slo-1 or kcnma1, bottom two traces. C, a summary of experiments conducted as shown in B. For each experiment, 10-min recording of basal pharyngeal pumping was acquired. This was subjected to analysis with AutoEPG (Dillon et al., 2009), which counted the number of pumps that occurred as single pumps and the number of pumps that occurred in groups of three or more. The data are expressed as the number of pumps that occurred in groups of one to three (open bars) or more than three (hatched bars) as a percentage of the total number of pumps. Data are the mean ± S.E.M. of n = 14 to 26. Note that the pattern of pumping in both the slo-1 and kcnma1 rescue lines is more like wild-type than slo-1(js379).