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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Mol Microbiol. 2011 Apr 11;80(4):1014–1030. doi: 10.1111/j.1365-2958.2011.07627.x

Fig. 1.

Fig. 1

(A) Scheme showing the role of Spo0A~P as a master upstream regulator of both eps expression and transcription of sporulation genes. Spo0A~P activates a promoter in front of sinI (Shafikhani et al., 2002). The eps operon is directly repressed by SinR (Kearns et al., 2005). Generally, repression due to SinR is lifted when it is sequestered by the protein SinI (Bai et al., 1993). (B) eps-lacZ expression in mecA and pKD93 strains. Wild-type (BD4498, empty squares), mecA::erm (BD4538, filled squares) and pKD93 (BD4644, filled circles) strains were grown in LB and β-galactosidase activities were determined at the indicated times. The triangles show results for strains growing in MsGG medium; wild-type (BD4498, closed triangles) and pKD93 (BD4644, open triangles). (C) spoIIG-luc expression in mecA and pKD93 strains. Wild-type (PP533, black line), mecA (PP551, gray line) and pKD93 (PP565, dotted line) strains were grown in DSM in a plate reader and growth (OD at 600 nm) and light output was measured every 1.5 minutes. For both panels, “Time” is given as hours before and after the transition to stationary phase.