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. Author manuscript; available in PMC: 2012 May 3.
Published in final edited form as: Biochemistry. 2011 Apr 8;50(17):3481–3494. doi: 10.1021/bi200165h

Figure 5.

Figure 5

SDS-PAGE analysis of PAc hydrolase autophosphorylation with [γ 32P]ATP. The top panel is the Coomassie blue stained gel and the bottom panel is the audioradiograph of the gel. Lane 1: Protein molecular weight standards. Lane 2: The reaction of [γ 32P]ATP with acid-denatured wild-type PAc hydrolase. Lane 3: The reaction of [γ 32P]ATP with wild-type PAc hydrolase. Lane 4: The reaction of [γ 32P]ATP with wild-type PAc hydrolase in the presence of 2 mM phosphonoformate. Lane 5: The reaction of [γ 32P]ATP with acid-denatured T64A PAc hydrolase. Lane 6: The reaction of [γ 32P]ATP with T64A PAc hydrolase. Lane 7: The reaction of [γ 32P]ATP with T64A PAc hydrolase in the presence of 2 mM phosphonoformate.