Table 4.
Oligonucleotide primer sets and PCR amplicon properties
| No | Gene* | Chromosome | Amplicon | Primer (5'-3') | ||
|---|---|---|---|---|---|---|
| Name | No. | Position (Start-End)** | %GC | Fragment (bp)/name | Forward/reverse | |
| Pr. 1 | Thy-1 | 9 | 43854065-43854933 | 53.6 | 864/Fr. 1 | ATGAACCCAGCCATCAGCG/GGGTAAGGACCTTGATATAGG |
| Pr. 2 | NTAL | 5 | 135085842-135086225 | 50.8 | 384/Fr. 2 | CTAGGGAGCTGAGTGTTCTCA/GAACGGCTAGAACTACACAGAG |
| Pr. 3 | NP_660313.1 | 16 | 613645-614375 | 71.5 | 731/Fr. 3 | GGTCGCCGACATCCACTC/TGCTCCGGGAACAGAACCT |
| Pr. 4 | Q8WZ58 | 11 | 2292033-2292813 | 71.7 | 781/Fr. 4 | CCTGACCGTCCTGGCACA/CTGGCGAAATCTGCGAGTTC |
| Pr. 5 | NP_060193.2 | 9 | 140174905-140175662 | 72.4 | 758/Fr. 5 | CCCCTCACTCAGGTCGTGTTTT/CCCTCTGAGCCCCTTTCG |
| Pr. 6 | Q8N4X1 | 16 | 2029023-2029774 | 72.6 | 752/Fr. 6 | CGGTCCATCCCCTCATCG/ACCCCTCACGCCACCAC |
| Pr. 7 | NP_001035158.1 | 16 | 33961629-33962363 | 73.1 | 735/Fr. 7 | CGGCGAACCGGACATCC/GGCTCGTGAGGCGGGTCT |
| Pr. 8 | Q8N1R6 | 13 | 111267817-111268622 | 73.3 | 806/Fr. 8 | GACACGGCCCTGCTCC/GGGTGTGATTGAGCGAGTTG |
| Pr. 9 | NM_020975 | 10 | 43572333-43572724 | 79.1 | 392/Fr. 9 | CCCGCACTGAGCTCCTACAC/GGACGTCGCCTTCGCCATCG |
* Homo_sapiens LATESTGP database was used, except for Thy-1 and NTAL for which Mus_musculus LATESTGP database was used.
** Primer sets Pr. 3 - Pr. 9. are based on Ensembl release 56 - Sept 2009