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. 2011 Apr 13;4:16. doi: 10.1186/1756-6606-4-16

Figure 5.

Figure 5

Gap junction-incompetent UNC-7 maintains its functional interaction with NCA. (A) Representative evoked synaptic responses (upper traces) and quantification (lower plot) of animals of various genotypes relative to that of wild type (wt) animals; significance tests were carried out in comparison to that of wt. Currents were recorded from animals expressing wild-type and mutant-form of UNC-7 constructs (+) in the nca(lf); unc-7 triple mutants, and their non-transgenic sibling from the same parents (-) were examined as controls. UNC-7 expression fully reverted nca(lf); unc-7 evoked responses to the nca(lf) level. Expression of Cys-less or C191A UNC-7 also reverted the evoked response. (B) Representative traces of spontaneous mPSCs from wild type (wt), nca(lf) and nca(lf); unc-7 animals, expressing various UNC-7 constructs by the pan-neuronal promoter. (C) Quantification of the frequency (upper plot) and amplitude (lower plot) of mPSCs from animals of various genetic backgrounds shown in (B). Transgenic animals expressing various UNC-7 constructs (+) and their non-transgenic siblings from the same parent as controls (-), were quantified by Student's t-test. * p < 0.05, ** p < 0.01, *** p < 0.001.