Table 1.
Minimal inhibition concentrations (MICs) and minimal microbicidal concentrations (MMCs) of def1 and def2 isoforms.
| Bacterial, yeast and viral species | MIC [μM] | MMC [μM] | ||
|---|---|---|---|---|
| def1 | def2 | def1 | def2 | |
| Bacillus subtilis (G+) | 1.5 | 0.75 | 2 | 1 |
| Micrococcus luteus (G+) | 0.75 | 0.37 | 10 | 5 |
| Staphylococcus luteus (G+) | 50 | 25 | 50 | 25 |
| Escherichia coli (G-) | no effecta | no effecta | ||
| Pseudomonas aeruginosa (G-) | no effectb | no effectb | ||
| Candida albicans (yeast) | no effectb | no effectb | ||
| TBEV (Flaviviridae) | no effectb | no effectb | no effectb | no effectb |
| WNV (Flaviviridae) | no effectb | no effectb | no effectb | no effectb |
Determination of MICs and MMCs for each peptide and bacteria was performed at least 3-times in doublets. Def2 peptide was significantly more efficient in the inhibition of growing G-positive bacteria (G+) in cultures and in microbicidal experiments. Gram negative bacteria (G-), as well as yeast, WNV and two strains of TBEV (Hypr and Neudoerfl), were not influenced by these peptides (a no effect up to 50 μM; b no effect up to 100 μM).