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. 2011 May 26;6(5):e19664. doi: 10.1371/journal.pone.0019664

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A) Tim-3 and IL-12 expression in THP-1 cells treated with C1q (0, 50, and 100 µg/ml) for 48∼72 h. Representative histogram over-layed at 72 h is shown above, and summary data from 3 independent experiments is shown below. B) Regulation of Tim-3 and IL-12 expression in M/M_Ø by HCV core protein in a gC1qR-dependent manner. PBMC were pre-incubated with anti-gC1qR or control serum overnight, followed by the stimulation with LPS/R848 in the presence of HCV core protein for 18 h. Cell surface Tim-3 and intracellular IL-12 expression was detected by flow cytometry. Data are reproducible in 6 independent experiments.