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. 2011 May 26;6(5):e18718. doi: 10.1371/journal.pone.0018718

Figure 2. Insertional mutagenesis of the spkC and spkG genes in Synechocystis PCC6803.

Figure 2

(A) Schematic representation of the constructs used to generate ΔspkC and ΔspkG mutants. The above arrows indicate directions of transcription. The restrictive sites were all added at the end of the primers, as shown in Table 1. Primers of T1 and T2, same with those for RT-PCR, are used for PCR determination of completely mutant separation. (B) PCR determination of the complete separation of ΔspkC and ΔspkG mutants. M, DL2000 marker.