Table 1. Oligonucleotide sequences used in this study.
| Primer | Sequences(5′-3′) | Restrictive sites |
| Mutant construction | ||
| spkC(slr0599) | ||
| Upstream | TTGAATTCGTTACCCCACTCAAAC | EcoRI |
| ATGGATCCCTTGGTCTATGACCTT | BamHI | |
| Downstream | ATGTCGACTTGCTAATGGCAAGAC | SalI |
| AATAAGCTTCCCTAATTTTGCTCGG | HindIII | |
| spkG(slr0152) | ||
| Upstream | CAGAATTCATGGTAAAGACTGCCA | EcoRI |
| TAGGATCCCTTTGGGGTAATTTCT | BamHI | |
| Downstream | ACGTCGACAAGTAAATCAAGACCA | SalI |
| ACCAAGCTTAATGTTTTCCACTTGG | HindIII | |
| kna | TCGGATCCAGTCAGCAACCATAGT | BamHI |
| ATGTCGACCCGCTCAGAAGAACT | SalI | |
| RT-PCR | ||
| spkC(slr0599) | CAGTTTGGGACTAACGGC | |
| TAAACCTTGGTGGCTTGG | ||
| spkD(sll0776) | CACTAGGGGATTTATGG | |
| TTGGTGGAACTTCTCGT | ||
| spkF(slr1225) | TCTTAGTTTCGTCCACGG | |
| ACCCTGATTGCCTTTACC | ||
| spkG(slr0152) | TTACTTGCCCCCATTGT | |
| CTCCCTGGATTAAGAGGC | ||
| rnp(slr1469) | GGACTACCCAAAACACTGC | |
| CAATAATCCCAGCTTGGCT |