Figure 3. Transduction of CHO-K1 or CHO-2241 cells by GFP-expressing, fiber mutants of HAdV5-based vectors in the absence (w/o) or presence of (with) FX (8 µg/ml).

(A), HAdV5wt, mutants HAdV5F^TTAT and HAdV5Pb^EGD and serotype 35 fiber-pseudotyped HAdV5F35 were all used at MOI 2,500, and transduction efficiency were expressed as arbitrary units (AU), as described in the legend to Fig. 2. (B), Dose-response effect of FX on cell transduction by HAdV5F^TTAT mutant. CHO-K1 cells were transduced by GFP-expressing HAdV5F^TTAT mutant vector in the presence of increasing concentration of FX (FX_max = 8 µg/ml). Results were expressed as relative transduction efficiency (RTE; refer to the legend to Fig. 2).