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. 2011 May 26;6(5):e17711. doi: 10.1371/journal.pone.0017711

Figure 8. Treatment of LCL C0913 with AzadC.

Figure 8

A: The ASM of LCL C0913 is shown before and following treatment with 5 µM AzadC. Mean methylation levels decreased from 20.7±4.9% to 10.2±2.8% for the GT allele and from 69±5.4% to 15.7±4.9% for the AC allele. Mean methylation level in the control cells did not change, that is, GT allele showed 22.5±5.8% and the AC allele 71.1±10.9%. B: ASE of LCL C0913 subjected to AzadC treatment. Allele frequencies were obtained by pyrosequencing of cDNA. Untreated cells showed allelic, that is more expression from the less methylated GT allele. By contrast, the allele frequency of the GT allele diminished from 71.6±2.8% to 49.9±2.3% following AzadC treatment. The control cells showed a GT allele frequency of 69.9±6.5%.