Figure 2. The operation of a novel “Double-Strips 2D BN/SDS-PAGE” transfer technique from the first dimension to the second.

A: One strip of 1D BN-gel lane was placed in the glass plate after denaturing. B: The other identical BN-gel strip was covered to the previous according to the aligned protein complexes, and then the glass plate was overlapped on the gels. C: The cassette was placed in skew form avoiding air bubble produced, the agarose was poured in the space between the BN-gel and stacking gel (just the half of all volume, as arrow indicated) after acrylamide was polymerized. D: The remaining space was subsequently filled with agarose until the previous solution has been concreted.