Figure 3. Kinetics of alanine racemase and L-alanine dehydrogenase activities in HTS format.

(A) When converted to 384-well plate format, the alanine racemase reaction gives a linear increase in fluorescence over a 40-minute period (⧫) as compared to the background control without substrate (▴). (B) The L-alanine dehydrogenase reaction gives a linear increase in fluorescence over a 20-minute period (▪) as compared to the background control without substrate (⧫).