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. 2011 May 26;6(5):e20480. doi: 10.1371/journal.pone.0020480

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Vrana et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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a) Collation of a full surface of a circular PLLA/Ti hybrid implant, seeded with freshly isolated Human respiratory epithelial cells isolated from nasal polyps(Scale bar: 500 µm). c) DAPI and anti-ZO1 stainings showed monolayer formation on the hybrid implant surface (Day 19). c) Confocal images on x–y plane and Z-section confirms the development of strong cell-cell contacts. d) SEM image of the overall hybrid structure, showing the titanium beads, the micropous body and the top film layer.