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. 2011 May 26;7(5):e1002071. doi: 10.1371/journal.pgen.1002071

Figure 2. Expression analysis of different Hoxd genes in control and HoxC mutant mice.

Figure 2

(A) Schematic representation of the wild type and the HoxC deleted allele. (B) Absolute and relative quantifications of posterior Hoxd genes transcripts and of mHotair in forebody, hindbody, forelimbs and hindlimbs of E13.5 embryos. All values are normalized to a housekeeping gene. Relative amounts were calculated as a ratio by forcing wild type values to 1. Accordingly, small values are over-represented, explaining why mHotair gives a signal after deletion of HoxC, even though it is obviously absent. (C) Whole mount in situ hybridization (WISH) of Hoxd10 on E12.5 developing embryos. The expression domains of Hoxd genes remain globally unchanged (D) Hoxd10 expression patterns in developing forelimbs and hindlimbs at three developmental stages. Expression domains of Hoxd genes remain globally unchanged at all stages of limb development examined.