Fig. 1.
Recruitment of β-arrestin-1 by the agonist-activated full-length wild-type corticotropin-releasing factor type 1 (CRF1) receptor in transfected HEK293 cells. Confocal microscopy was used to evaluate the interaction of β-arrestin-1-green fluorescent protein (GFP) with full-length CRF1 receptors transiently expressed in HEK293 cells. This representative experiment shows the distribution of β-arrestin-1-GFP in HEK293 cells before (0 min) and after (2, 5, and 20 min) treatment with 200 nM CRF.