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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Nat Genet. 2011 May 1;43(6):595–600. doi: 10.1038/ng.830

Figure 3.

Figure 3

Confocal microscopy was performed using HeLa cells co-transfected with plasmids containing RFP-PCNA and GFP-DNMT1 wild type TS domain (panel a), p.Tyr495Cys-TS domain (panel b) or p.Asp490Glu-Pro491Tyr-TS domain (panel c). Wild type and mutant TS domains appear green in the right panels, PCNA appears red in the middle panels and merged images are shown in the left panels. Scale bar, 5um. In panel a, wild type TS domain enters nucleus during S phase when PCNA localizes to the toroidal structures of replication foci, wild type TS also binds to heterochromatin during G2 phase when PCNA showed diffused pattern and toroidal structures are no longer visible. Panel b and panel c showed mutant TS domains was unable to enter into the nucleus and remained in the cytoplasm.