FIG. 2.

Expression of CXCR4 and CXCR7 by mouse neural progenitor cells (mNPCs). (A) mNPCs are multipotent. Triple staining is shown for astrocytes (GFAP, blue), neurons (Beta-III tubulin, red), and oligodendrocytes (O4, green). (B) Floating neural spheres before trituration to single cell suspension. (C) After trituration, the single cell suspension was plated and expanded as NPCs. (D) RT-PCR for mRNAs of CXCR4, CXCR7, and glyceraldehyde 3-phosphate dehydrogenase. Bottom panels show no reverse transcriptase controls for each primer set. (E–G) Immunocytochemistry for CXCR4. (H–J) Isotype controls for CXCR4 (rabbit isotype). (K–M) Immunocytochemistry for CXCR7. (N–P) Isotype controls for CXCR7 (mouse isotype). Insets: close-up views of cell staining. Color images available online at www.liebertonline.com/scd